جامعة بلاد الرافدين - Bilad Alrafidain University - أ.م.د.احمد حسين قاسم

أ.م.د.احمد حسين قاسم

Assist.Prof.Dr. Ahmed Hussein Qasim

رئيس قسم : تقنيات العلاج الطبيعي

Head of the Department : Physical therapy techniques

دكتوراه في الطب

PhD in Medicine

Dr.ahmedQa@bauc14.edu.iq

ahmedarnaoty1977@gmail.com

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المقالات داخل المجلة

البحوث

	2022	Indian Journal of Forensic Medicine and Toxicology
Abstract DNA transposons are exposed to a molecular domestication process, which results in the formation of neogenes, which may play a role in human genetic instability. TIGD3 (Tigger-derived [TIGD] family of proteins) is one of these Neogene, and its role in the human genome is unknown. Aim: The expression of Neogene TIGD3 in colorectal cancer cell lines and its putative function in carcinogenesis are being investigated. Method: The protein expression of the TIGD3 gene was investigated using the western blot method in twelve colorectal cancer cell lines (HCT116, SW48, LOVO, DLD1) that are microsatellite instable MSI, (SW480, SW620, HT29, LS123, COLO205, T84, SW403, SW1463) that are microsatellite stable MSS, and in healthy colon tissue as a control in our study. Results: The expression of the TIGD3 protein was found in all twelve colorectal cancer cell lines, with varying degrees of expression and numerous isoforms, which was not found in healthy colon tissue. Conclusion: There may be a link between colorectal cancer evolution or progression and TIGD3 gene expression

	2023	Indian Journal of Forensic Medicine and Toxicology
Abstract Background: The objectives of this study were to: (1) find out the levels of thyroid hormones, lipid profile, and glycosylated hemoglobin (HbA1c) in diabetic patients type II; and (2) examine the relationship between thyroid hormones and HbA1c, as well as distinctive sorts of lipids and HbA1c in the patient groups. Materials and Methods: A retrospective chart review study for the group of patient’s vs control was carried out at Al-Yarmouk Teaching Hospital in Baghdad Al-Karkh, Iraq. From December 2020 to February 2022, 100 male and female patients with type II diabetes mellitus and 100 non- diabetic males and females as controls were included, respectively, in this study. The biochemical laboratory tests were obtained from a laboratory database of the hospital. Statistical analysis was performed using SPSS version 21 to estimate the P-value from the T-test of independent groups. Results: For the patient groups compared with the control groups, there was an increase in the mean levels of both HbA1c (7.84%) and TSH (7.65 μlU/ml), while T4 (10.31 μg/dl) and T3 (1.44 ng/ml) were normal. It also increased mean levels of triglycerides (191.46 mg/dl) and normal total cholesterol (185.94 mg/dl). The results of the patient groups showed an insignificant correlation between HbA1c and TSH (P = 0.96844) and a significant correlation between HbA1c with T4 and T3 (P = 0.00323) and (P = 0.00001) respectively. Significant and positive relationship between HbA1c and total cholesterol and triglycerides (P = 0.00001), (P = 0.00001) respectively. Conclusion: Increased blood glucose did not cause the anterior pituitary gland to enhance TSH production, although there was a clear link between increased glycemic index and the rate of thyroxin secretion. Furthermore, there is a link between blood glucose and several lipid markers, according to the findings.

	2022	IOSR Journal Of Pharmacy And Biological Sciences
Abstract The neogenic recombinases could be a source of genetic variety, and they could be involved in the pathways of genetic instability in carcinogenesis. The nuclear apoptosis-inducing factor 1 (NAIF1), which codes the protein NAIF1 that triggers apoptosis in several human malignancies, is one of these neogenic recombinases. The purpose of this research is to look at the expression of the NAIF1 neogene in seven different glioma cell lines (H4, HS683, 42-MG-BA, A172, U87MG, U118MG, SK-N-MC).The protein expression of the NAIF1 gene in samples of protein isolated from various glioma cell lines was investigated using the western blot method.The findings revealed that while NAIF1 protein expression was observed in all cell lines, it was moderately expressed in all primary non-metastatic cell lines and very weakly expressed in one cell line generated from a metastatic site, SK-N-MC.It can be concluded that NAIF1 protein expression is negatively related to advanced cancer stage or grade, and that it may have a role in suppression of cancer proliferation, migration, and invasion via inducing apoptosis.

	2021	Indian Journal of Forensic Medicine & Toxicology
AbstractObjective: To evaluate the association of metformin use with vitamin B12 deficiency in Iraqi patients with type II diabetes mellitus.Methods: This is a prospective study conducted among the diabetic patients coming to the outpatient clinic at Al-Zahraa teaching hospital in Al-Kut and Al-Baghdad teaching hospital in the medical city of Baghdad from October to December 2018, the samples taken randomly after taking a short history from each patient. Data concerning age, medical disease including diabetes mellitus, and medications including metformin (average daily dose and duration of use) at the time of vitamin B12 measurement were collected. Our patients were classified into 2 groups: diabetic patients taking metformin, diabetic patients not taking metformin. The statistical analysis was done using the SPSS 23 statistical package to analyze the data. The two groups were compared by Paired Samples t-test. Data are presented as the number, percentage, mean value, and standard deviation. The p-value < 0.05 was taken as significant.Results: Vitamin B12 level in diabetic patients who have been on metformin for ≤ 1 year at a dose of ≤ 1 gm/day showed a significant difference with those patients with no history of metformin use.Conclusion: Low levels of vitamin B12 are related to a longer period and a higher dose of metformin use.

	2021	Medico-legal Update
AbstractThe neogenic recombinases are potentially a source of genetic variability possibly implicated in the mechanisms of genetic instability involved in the processes of carcinogenesis. One of these neogenic recombinases is the nuclear apoptosis-inducing factor 1(NAIF1), which codes the protein NAIF1 that induces apoptosis in various human cancers.The aim of this work is to study the expression of NAIF1 neogene in cancerous and non-cancerous colorectal tissues.The protein expression of NAIF1gene has been studied by western blot method in the samples of protein extracted from 29 patients with colorectal cancer.The result of this study showed that the protein expression of NAIF1 was found in both cancerous and non-cancerous colorectal tissues, but it is highly expressed in non-cancerous tissues adjacent to cancerous one. Its expression is higher in non-metastatic cancerous tissues compared with metastatic one. Also this expression is higher in microsatellite instable (MSI) group of cancer compared with microsatellite stable (MSS) group.It can be concluded that NAIF1 protein expression inversely related with more advanced stage or grade of colorectal cancer and it may have a role in inhibition of proliferation, migration and invasion of colorectal cancer by inducing apoptosis

	2017	AJPS
Abstract The process of molecular domestication which had been occured in some DNA transposons (2nd class of transposable elements) led to the formation of novel genes, which are called “Neogenes”. These neogenes may play an important role in the human genetic instability, human diseases and cancer. One of these Neogene is nuclear apoptosis-inducing factor 1(NAIF1) which inducing apoptosis in various human cancers. The aim of this work is to study the expression of NAIF1 Neogene in leukaemia cell lines. The protein expression of NAIF1gene has been studied by western blot method in seven leu-kaemia cell lines (HL60, NB4, KG1, KG1a, ML2, THP1 and U937) and in a healthy tissue of blood as a control. The result of this study showed that the protein expression of NAIF1 in leukaemia cell lines were found in variable degree of expression, which was highly expressed in healthy blood tis-sue. From the results of this work, it can be concluded that NAIF1 protein may have a role in inhi-bition of proliferation, migration and invasion of leukaemia by inducing apoptosis, which need further research and confirmation.

	2016	AJPS
bstract:The process of molecular domestication that occurred on DNA transposons and gives what is called neogenes that may play an important role in the human genetic instability. One of these Neogene is piggyBac 3 (PGBD3) which is associated with human Cockayne syndrome and Premature ovarian failure. The aim of this work is to study the expression of Neogene PGBD3 in colorectal cancer cell lines.The protein expression of PGBD3 gene have been studied by western blot method in twelve colorectal cancer cell lines (HCT116, SW48, LOVO, DLD1) which are microsatellite instable MSI, (SW480, SW620, HT29, LS123, COLO205, T84, SW403, SW1463) which are microsatellite stable MSS and in healthy tissue of colon as a control. The result of this study showed that the protein expression of PGBD3 gene in all 12 colorectal cancer cell lines were obtained with variable degree of expression which was not seen in healthy colon tissue. From the results of this work,it can be concluded that PGBD3 may have a role in colorectal cancer either in initiation, promotion or progression which need further research and confirmation.

	2017	AJPS
AbstractThe process of molecular domestication which had been occured in some DNA transposons (2nd class of transposable elements) led to the formation of novel genes, which are called “Neogenes”. These neogenes may play an important role in the human genetic instability, human diseases and cancer. One of these Neogene is nuclear apoptosis-inducing factor 1(NAIF1) which inducing apoptosis in various human cancers. The aim of this work is to study the expression of NAIF1 Neogene in leukaemia cell lines.The protein expression of NAIF1gene has been studied by western blot method in seven leu-kaemia cell lines (HL60, NB4, KG1, KG1a, ML2, THP1 and U937) and in a healthy tissue of blood as a control.The result of this study showed that the protein expression of NAIF1 in leukaemia cell lines were found in variable degree of expression, which was highly expressed in healthy blood tis-sue.From the results of this work, it can be concluded that NAIF1 protein may have a role in inhi-bition of proliferation, migration and invasion of leukaemia by inducing apoptosis, which need further research and confirmation

	2022	International Journal of Applied Sciences and Technology
Abstract:Background: In advanced human malignancies, the nuclear apoptosis-inducing factor 1 (NAIF1) is commonly silenced or not expressed. The purpose of this study was to look into potential relationships between clinical, pathological characteristics and NAIF1 expression.Methods: This cross-sectional study used western blot and immunohistochemistry staining to assess the level of NAIF1 expression in 100 colorectal cancer samples. The outcomes of the immunohistochemistry staining were then contrasted with those of the clinicopathological characteristics.Results: 68 out of 100 colorectal cancer samples were negative for NAIF1 expression.In contrast to other clinicopathological parameters, loss of NAIF1 expression was significantly linked with lymphovascular invasion (P=< 0.00001), angiovascular invasion (P= 0.004356) andadvanced TNM tumor stage (P=0. 004077).Conclusion: According to the current study, a higher stage and a bad prognosis may be linked to decreased or negative NAIF1 expression

	2012	Methods Mol Biol
Abstract Molecular domestication of several DNA transposons has occurred during the evolution of the primate lineage, and has led to the emergence of at least 42 new genes known as neogenes. Because these genes are derived from transposons, they encode proteins that are related to certain recombinases, known as transposases. Consequently, they may make an important contribution to the genetic instability of some human cells. In order to investigate the role of these neogenes, we need to be able to study their expression as proteins, for example in tumours, which often provide good models of genetic instability. In order to perform such studies, polyclonal antibodies directed against the proteins expressed by neogenes are obtained using a recently developed new method of Nanospheres/DNA immunisation in laboratory mammals. In this chapter, we describe a fully integrated process of producing antibodies that consists of a series of steps starting with the preparation and synthetic formulation of plasmids encoding neogenes, and culminating in the final production and confirmation of the quality of these polyclonal antibodies.

	2013	Mol.Gen.Gent
Abstract The molecular domestication of several DNA transposons that occurred during the evolution of the mammalian lineage, has led to the emergence of at least 43 genes, known as neogenes. To date, the limited availability of efficient commercial antibodies directed against most of their protein isoforms hampers investigation of their expression in vitro and in situ. Since immunization protocols using peptides or recombinant proteins have revealed that it is difficult to recover antibodies, we planned to produce antisera in mice using a new technique of nanopheres/DNA immunization, the ICANtibodies™ technology. Here, we investigate the possibilities of obtaining polyclonal antibodies for 24 proteins or protein domains using this immunization strategy. We successfully obtained 13 antisera that were able to detect neogenic proteins by Western blotting and ELISA in protein extracts of transiently-transfected cells and various cancer cell lines, plus another two that only detected the in ELISA and in in situ hybridizations. The features required for the production of these antibodies are analyzed and discussed, and examples are given of the advantages they offer for the study of neogenic proteins.

	2013	Gene
Abstract Deciphering the mechanisms underlying the regulation of DNA transposons might be central to understanding their function and dynamics in genomes. From results obtained under artificial experimental conditions, it has been proposed that some DNA transposons self-regulate their activity via overproduction inhibition (OPI), a mechanism by which transposition activity is down-regulated when the transposase is overconcentrated in cells. However, numerous studies have given contradictory results depending on the experimental conditions. Moreover, we do not know in which cellular compartment this phenomenon takes place, or whether transposases assemble to form dense foci when they are highly expressed in cells. In the present review, we focus on investigating the data available about eukaryotic transposons to explain the mechanisms underlying OPI. Data in the literature indicate that members of the IS630-Tc1-mariner, Hobo-Ac-Tam, and piggyBac superfamilies are able to use OPI to self-regulate their transposition activity in vivo in most eukaryotic cells, and that some of them are able to assemble so as to form higher order soluble oligomers. We also investigated the localization and behavior of GFP-fused transposases belonging to the mariner, Tc1-like, and piggyBac families, investigating their ability to aggregate in cells when they are overexpressed. Transposases are able to form dense foci when they are highly expressed. Moreover, the cellular compartments in which these foci are concentrated depend on the transposase, and on its expression. The data presented here suggest that sequestration in cytoplasmic or nucleoplasmic foci, or within the nucleoli, might protect the genome against the potentially genotoxic effects of the non-specific nuclease activities of eukaryotic transposases.

	2021	Genomics
Abstract Setmar is a gene specific to simian genomes. The function(s) of its isoforms are poorly understood and their existence in healthy tissues remains to be validated. Here we profiled SETMAR expression and its genome-wide binding landscape in colon tissue. We found isoforms V3 and V6 in healthy and tumour colon tissues as well as incell lines. In two colorectal cell lines SETMAR binds to several thousand Hsmar1 and MADE1 terminal ends, transposons mostly located in non-genic regions of active chromatin including in enhancers. It also binds to a 12-bp motifs similar to an inner motif in Hsmar1 and MADE1 terminal ends. This motif is interspersed throughout the genome and is enriched in GC-rich regions as well as in CpG islands that contain constitutive replication origins. It is also found in enhancers other than those associated with Hsmar1 and MADE1. The role of SETMAR in the expression of genes, DNA replication and in DNA repair are discussed.

	2024	Oncologyand Radiotherapy
Background: Advanced human cancers exhibit significant levels of TIGD3 expression. The purpose of this study was to look at possible associations between positive TIGD3 expression and clinical, pathological aspect in colorectal cancer patients. Methods: In this cross-sectional investigation, TIGD3 expression levels were measured in 100 colorectal cancer tissues using western blot and immunohistochemical labeling. Following that, immunohistochemical-staining outcomes were contrasted with clinicopathological characteristics. Results: In 82 out of 100 colorectal cancer samples, TIGD3 expression was shown to be present. TIGD3 expression was substantially correlated with higher stages of cancer TNM III-IV (p=000117), lymph vascular invasion (p=000045) and angiovascular invasion (p=00001), in contrast to other clinicopathological criteria. Conclusion: The results of this investigation imply that elevated or positive TIGD3 expression may be linked to an advanced stage and a bad prognosis.

المؤلفات

	2024	DSNC

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